Staff Research Associate III - Ophthalmology

Under the
direction of the Principal Investigator (PI), the Staff Research Associate III
(SRA III) is responsible for executing cellular and molecular techniques as
part of the basic, translational and clinical research. Duties include the cell
culture of primary cells and cell lines as well as other molecular biology
techniques including but not limited to cloning, mutagenesis, sequencing,
polymerase chain reaction (PCR), Western Blot, immunohistochemistry/immunocytochemistry,
and cryosectioning. The SRA III will perform imaging acquisition and imaging
and data analysis, keep good documentation records in compliance, assist with
pre-clinical experiments and actively participate in qualifications and
clinical manufacturing part of a Phase I clinical trial.

The SRA III
will also be in charge of lab management and lab maintenance including but not
limited to overseeing lab activities ensuring that a safe, clean, organized,
well equipped/stocked and compliant environment is maintained.

All
Required:

• Bachelor's
  degree in life science (not including psychology) and or equivalent experience
  and/or training
• 
  Knowledge of
  molecular biology techniques
• 
  Skills and
  extensive experience in human and mouse cell culture technique
• 
  Knowledge of
  cell culture, including thawing cells, culturing cells and freezing cells
• 
  Skills in
  cryosection
• 
  Skills in
  isolating and purifying high quality DNA and RNA
• 
  Skill in
  subcloning of cell lines
• 
  Ability to use
  molecular biology software
• 
  Ability and
  experience to perform immunohistochemistry
• 
  Ability to
  perform protein analysis by gel electrophoresis and Western Blots
• 
  Experience with
  tissue and cell imaging and analysis
• 
  Ability to
  establish and maintain records and supply stocks
• 
  Skill in
  maintaining the lab and equipment in working condition
• 
  Ability to
  establish and maintain cooperative working relationships with co-workers
• 
  Demonstrated
  experience using general lab equipment such as pipettes, centrifuges,
  balances,incubators, thermal cyclers, dry blocks, shakers, autoclaves,
  water baths, liquid nitrogen tanks,DNA synthesizer, pH meter, etc.
• 
  Ability to work
  with mice, setting up breeding pairs and recording births and weaning animals
• 
  Ability to
  plan, execute and coordinate all the GMP/GLP activities related to the
  pre-clinicaland Phase I clinical projects in collaboration with the
  research teams
• 
  Knowledge of
  GLP and GMP
• 
  Ability to
  manage and maintain the lab ensuring that a safe, clean and compliant
  environment ismaintained
• 
  Ability to work
  on multiple projects, often changing from one task to another
• 
  Ability to
  establish and maintain cooperative working relationships with personnel both in
  andoutside of the laboratory group
• 
  Communicate
  clearly, concisely and accurately in verbal and written formats
• 
  Ability to
  summarize and present data