Laboratory Technician

The Department of Neurobiology is seeking a motivated Lab Technician to join our research team. In this role, you will contribute to neuroscience research by making and maintaining primary neuronal cultures and performing routine molecular biology techniques, including DNA cloning and PCR. Responsibilities also include assisting with the growth and maintenance of mouse and human pluripotent stem cell lines, as well as supporting neuronal differentiation experiments such as brain organoid formation and maintenance. You will help with fixation, embedding, cryostat sectioning, and immunohistochemical staining of brain organoids and two-dimensional cultures, and will assist in the production and use of non-pathogenic lentiviruses and adeno-associated viruses. Additional duties include preparing cells for flow cytometry and fluorescence-activated cell sorting, collecting images using fluorescence and confocal microscopy, and preparing organoid and cell culture samples for single-cell or single-nucleus sequencing. This position offers an excellent opportunity to gain hands-on experience with advanced neurobiological methods and contribute to innovative research in neural development and disease modeling.

Hourly range: $23.12-$27.20

Required:

• Skills in Photoshop and Illustrator, and familiarity with microscopy image collection and processing software applications such as ImageJ, Axiovision, Zen, and Imaris, and statistical analysis programs such as Prism.
• Demonstrated skills in mammalian tissue culture.
• Demonstrated skills working with human pluripotent stem cells.
• Demonstrated ability to work with accuracy and attention to detail. Strong organizational abilities. Able to maintain complete and accurate records.

• Demonstrated skills in conducting cellular differentiation experiments (organoid and non-organoid).
• Demonstrated skills in tissue collection, fixation, processing, and cryosectioning.
• Demonstrated skills in conventional and confocal microscopy
• Demonstrated skills in molecular biology (DNA cloning, PCR, plasmid preparation, DNA and RNA extraction.
• BA/BS in neuroscience, molecular biology, developmental biology or equivalent.